1
Cryopreservation
2
3
Nalgene “Mr Frosty”
4
5
6
Fundamental cryopreservation protocol
7
Cryopreservation
8
containers
9
Optimal results for cryopreserved oocytes
10
Worst results for cryopreserved oocytes
11
Cryopreservation of human oocytes
12
13
Cryopreservation of
unfertilized oocytes
14
15
16
Ice crystal size v.s. water content
17
Dehydration without protection
18
19
Disruption to the metaphase spindle
20
21
In vs. out.
22
Ethylene glycol Mol. Wt. 62.07
= Ethanediol (C
2
H
6
O
2
)
23
24
25
26
27
28
29
30
31
Return to the
Hygiene and Husbandry Conference
presentations.
